pAV0286
(Plasmid #149333)

• Purpose: Bacterial expression of SENP_EuB protease
• Depositing Lab: Dirk Görlich
• Publication: Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020. doi: 10.1083/jcb.201812091. Epub 2019 Apr 25.

Backbone

• Vector backbone: pDG-Kan
• Backbone manufacturer: Görlich lab
• Backbone size w/o insert (bp): 3997
• Total vector size (bp): 4720
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SENP_EuB protease
• Species: Synthetic
• Insert Size (bp): 723
• Tags / Fusion Proteins:
  • His14 (N terminal on insert)
  • Tev protease cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: pQE-for
• 3′ sequencing primer: pQE-rev

Resource Information

• Supplemental Documents:
  • pAV0286 H14-Tev-SENP1_EuB.gbk
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry