pTP264
(Plasmid #149334)

• Purpose: Bacterial expression of Biotin ligase BirA
• Depositing Lab: Dirk Görlich
• Publication: Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554/eLife.11349.

Backbone

• Vector backbone: pDG-Kan
• Backbone manufacturer: Görlich lab
• Backbone size w/o insert (bp): 4278
• Total vector size (bp): 5243
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Biotin ligase BirA
• Species: E. coli
• Insert Size (bp): 965
• Tags / Fusion Proteins:
  • His14 (N terminal on insert)
  • bdNEDD8 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: pQE-for
• 3′ sequencing primer: pQE-rev

Resource Information

• Supplemental Documents:
  • pTP264_H14-bdNEDD8-BirA.gbk
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry