168,037 results

1. pLJC5-Tmem192-2xFlag

   Plasmid

   #102929

   Purpose

   Lentiviral expression of a lysosomal tag (Tmem192-Flag)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   Tmem192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDF0159 pCMV - huDisCas7-11 mammalian expression

   Plasmid

   #172507

   Purpose

   Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pCMV - huDisCas7-11 mammalian expression
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV8)

   Viral Prep

   #50454-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (#50454). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, excitatory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCitrine (Cre-dependent)

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

4. TK4-EGFP

   Plasmid

   #239047

   Purpose

   PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

   Depositor

   Marius Wernig

   Inserts

   EGFP
   puroR
   

   Expression

   Mammalian

   Promoter

   CAG and EF1a

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-MAPPER

   Plasmid

   #117721

   Purpose

   MAPPER Selectively Labels ER-PM Junctions

   Depositor

   Jen Liou

   Article

   Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

   Insert

   MAPPER
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTripZ-Tet-ON mCherry

   Plasmid

   #187262

   Purpose

   Doxycyclin inducible expression of mCherry

   Depositor

   Alpha Yap

   Article

   Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

   Insert

   mCherry-NLS
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Sec16A

   Plasmid

   #36155

   Purpose

   Expresses Sec16A as a GFP fusion

   Depositor

   David Stephens

   Article

   Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

   Insert

   Sec16A (SEC16A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCHAC-mt-mKeima

   Plasmid

   #72342

   Purpose

   retrovirus construct for stable expressing mt-mKeima

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   mt-mKeima
   

   Use

   Retroviral

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TRE-dCas9-Zim3-MeCP2

   Plasmid

   #220143

   Purpose

   doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

   Depositor

   Frank Buchholz

   Article

   Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

   Insert

   dCas9-Zim3-MeCP2
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   Tre-3G

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Tags

    TurboRFP

    Expression

    Mammalian

    Promoter

    13X repeated p21-p53 response element

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGM4723

    Plasmid

    #48015

    Purpose

    Please see depositor comments section.

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. GBX

    Plasmid

    #64123

    Purpose

    a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    GFP-2A-BCL2L1 (BCL2 Human)
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPGK-T7/2-CD44s (fl)

    Plasmid

    #137812

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length, I186T and I253V on CD44

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

    Plasmid

    #167582

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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