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Roger Tsien Lab Plasmids


The Roger Tsien Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Palmer AE, Jin C, Reed JC, Tsien RY Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec 7.
Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, Tsien RY Chem Biol. 2006 May;13(5):521-30.
Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, Ellisman MH, Tsien RY, Lin MZ Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28.
TimeSTAMP tagging of newly synthesized proteins. Lin MZ, Tsien RY Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 10.1002/0471140864.ps2605s59.
Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, Tsien RY Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
Characterization of engineered channelrhodopsin variants with improved properties and kinetics. Lin JY, Lin MZ, Steinbach P, Tsien RY Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Lin JY, Knutsen PM, Muller A, Kleinfeld D, Tsien RY Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1.
Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, Lowy DR, Schiller JT Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
Michael Davidson Empty Backbones (unpublished)
A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21.
A monomeric red fluorescent protein. Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.
Understanding, improving and using green fluorescent proteins. Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY Trends Biochem Sci. 1995 Nov;20(11):448-55.
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31.
Improving the photostability of bright monomeric orange and red fluorescent proteins. Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4.
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
Evolution of new nonantibody proteins via iterative somatic hypermutation. Wang L, Jackson WC, Steinbach PA, Tsien RY Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov 19.
Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
36323pcDNA-D3cpvD3cpv second generation cameleon (calcium sensor) (Synthetic)Mammalian ExpressionCa2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. pcDNA3' Add to Cart
36324pcDNA-4mtD3cpv4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria (Synthetic)Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. pcDNA3 Add to Cart
36325pcDNA-D1ERD1ER second generation cameleon (calcium sensor) targeted to ER (Synthetic)Mammalian ExpressionBcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec 7. pcDNA3 Add to Cart
37470pBAD-D2D2 second generation cameleon (calcium sensor) (Synthetic)Bacterial ExpressionCa2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. pBAD Add to Cart
37471pcDNA-D2cpVD2cpv second generation cameleon (calcium sensor) (Synthetic)Mammalian ExpressionCa2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. pcDNA3' Add to Cart
37472pcDNA-lynD3cpVD3cpv second generation cameleon (calcium sensor) (Synthetic)Mammalian ExpressionCa2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. pcDNA3' Add to Cart
37473pBAD-D4D4 second generation cameleon (calcium sensor) (Synthetic)Bacterial ExpressionCa2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. pBAD Add to Cart
37479pcDNA-D1cpVD1cpV second generation cameleon (calcium sensor) (Synthetic)Mammalian ExpressionBcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec 7. pcDNA3' Add to Cart
37480pcDNA-D1D1 second generation cameleon (calcium sensor) (Synthetic)Mammalian ExpressionBcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec 7. pcDNA3' Add to Cart
42225PSD95-TS:YFPPSD95Mammalian ExpressionFluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. pCAGGS Add to Cart
42226PSD95-TS:YSOG1PSD95Mammalian ExpressionFluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. pCAGGS Add to Cart
42227PSD95-TS:YSOG2PSD95Mammalian ExpressionFluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. pCAGGS Add to Cart
42235pCS6-mPSD95a-TSa-HAPSD95Mammalian ExpressionTimeSTAMP tagging of newly synthesized proteins. Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 10.1002/0471140864.ps2605s59. TimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis rates pCS6 Add to Cart
42237pCS6-mPSD95a-TSt-HAPSD95Mammalian ExpressionTimeSTAMP tagging of newly synthesized proteins. Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 10.1002/0471140864.ps2605s59. TimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis rates pCS6 Add to Cart
42240pCS6-mPSD95a-TS2-HAPSD95Mammalian ExpressionFluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. pCS6 Add to Cart
48735ptwBTandem dual tomato (Other)Mammalian ExpressionGenital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1. Tandem dual tomato plasmid custom Add to Cart
50954AAV-ReaChR-citrineReaChR-citrine (Synthetic)AAVReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1. ReaChR-citrine in AAV2 vector under human synapsin promoter AAV2 Add to Cart
50955AAV-flex-ReaChR-citrineReaChR-citrine (Synthetic)AAVReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1. flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter AAV2 Add to Cart
50956pLenti-ReaChR-citrineReaChR-citrine (Synthetic)LentiviralReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1. ReaChR-citrine in lentival transfer vector under human synapsin promoter AAV2 Add to Cart
50969AAV-miniSOG-VAMP2-citrineminiSOG-VAMP2-citrine (Mus musculus)AAVOptogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022. AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct AAV2 Add to Cart
50970AAV2-miniSOG-VAMP2-T2A-mCherryminiSOG-VAMP2-T2A-mCherry (Mus musculus)AAVOptogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022. AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct AAV2 Add to Cart
50971AAV-SYP1-miniSOG-citrineSynaptophysin-miniSOG-citrine (Rattus norvegicus)AAVOptogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022. AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct AAV2 Add to Cart
50972AAV-SYP1-miniSOG-T2A-mCherrySynaptophysin-miniSOG-T2A-mCherry (Rattus norvegicus)AAVOptogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022. AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct AAV2 Add to Cart
50973AAV-flex-oChIEF-citrineChIEF-citrine (Synthetic)AAVCharacterization of engineered channelrhodopsin variants with improved properties and kinetics. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR AAV2 Add to Cart
50974AAV-oChIEF-citrineChIEF-citrine (Synthetic)AAVCharacterization of engineered channelrhodopsin variants with improved properties and kinetics. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR AAV2 Add to Cart
50975AAV-oChEF-citrineChEF-citrine (Synthetic)AAVCharacterization of engineered channelrhodopsin variants with improved properties and kinetics. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. AAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITR AAV2 Add to Cart
50976AAV-oChD-citrineoChD-citrineAAVCharacterization of engineered channelrhodopsin variants with improved properties and kinetics. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR AAV2 Add to Cart
50977AAV-oChIEF-tdTomatoChIEF-tdTomato (Synthetic)AAVCharacterization of engineered channelrhodopsin variants with improved properties and kinetics. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR AAV2 Add to Cart
54531mOrange2-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 549, Emission: 565 mOrange2-pBAD
  • Tags / Fusion Proteins
    • mOrange2 (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54536mApple-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 568, Emission: 592 mApple-pBAD
  • Tags / Fusion Proteins
    • mApple (C terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54564mPlum-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 590, Emission: 649 mPlum-pBAD
  • Tags / Fusion Proteins
    • mPlum (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54568mOrange2-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 549, Emission: 565 mOrange2-N1
  • Tag / Fusion Protein
    • mOrange2 (C terminal on backbone)
  • Add to Cart
    54587mCitrine-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 516, Emission: 529 mCitrine-C1
  • Tag / Fusion Protein
    • mCitrine (N terminal on backbone)
  • Add to Cart
    54593Citrine-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 516, Emission: 529 Citrine-N1
  • Tag / Fusion Protein
    • Citrine (C terminal on backbone)
  • Add to Cart
    54594mCitrine-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 516, Emission: 529 mCitrine-N1
  • Tag / Fusion Protein
    • mCitrine (C terminal on backbone)
  • Add to Cart
    54630mCherry-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 587, Emission: 610 mCherry-pBAD
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54635mRFP1-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 584, Emission: 607 mRFP1-N1
  • Tag / Fusion Protein
    • mRFP1 (C terminal on backbone)
  • Add to Cart
    54642tdTomato-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 554, Emission: 581 tdTomato-N1
  • Tag / Fusion Protein
    • tdTomato (C terminal on backbone)
  • Add to Cart
    54644mStrawberry-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 574, Emission: 596 mStrawberry-N1
  • Tag / Fusion Protein
    • mStrawberry (C terminal on backbone)
  • Add to Cart
    54650mOrange2-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 549, Emission: 565 mOrange2-C1
  • Tag / Fusion Protein
    • mOrange2 (N terminal on backbone)
  • Add to Cart
    54667mRFP1-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 584, Emission: 607 mRFP1-pBAD
  • Tags / Fusion Proteins
    • mRFP1 (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54714mNeptune-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 600, Emission: 650 mNeptune-pBAD
  • Tags / Fusion Proteins
    • mNeptune (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54715Citrine-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 516, Emission: 529 Citrine-C1
  • Tag / Fusion Protein
    • Citrine (N terminal on backbone)
  • Add to Cart
    54723mCitrine-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 516, Emission: 529 mCitrine-pBAD
  • Tags / Fusion Proteins
    • mCitrine (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54764mRFP1-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 584, Emission: 607 mRFP1-C1
  • Tag / Fusion Protein
    • mRFP1 (N terminal on backbone)
  • Add to Cart
    54772Citrine-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 516, Emission: 529 Citrine-pBAD
  • Tags / Fusion Proteins
    • Citrine (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54782IFP1.4-C1 Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 684, Emission: 708 IFP1.4-C1
  • Tag / Fusion Protein
    • IFP1.4 (N terminal on backbone)
  • Add to Cart
    54783IFP1.4-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 684, Emission: 708 IFP1.4-N1
  • Tag / Fusion Protein
    • IFP1.4 (C terminal on backbone)
  • Add to Cart
    54856tdTomato-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 554, Emission: 581 tdTomato-pBAD
  • Tags / Fusion Proteins
    • tdTomato (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    80341pBAD-smURFP-RBS-HO-1smURFP (Synthetic), HO-1 (Other)Bacterial ExpressionA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Expresses smURFP and Heme Oxygenase-1 in bacterial cells. pBAD Add to Cart
    80342pBAD-TDsmURFP-RBS-HO-1Tandem Dimer smURFP (Synthetic), HO-1 (Other)Bacterial ExpressionA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells. pBAD Add to Cart
    80343pcDNA3-smURFP-IRES-eGFPsmURFP (Synthetic), eGFP (Synthetic)Mammalian ExpressionA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Expresses smURFP and eGFP in mammalian cells. pcDNA3 Add to Cart
    80344pcDNA3-TDsmURFP-IRES-eGFPTandem Dimer smURFP (Synthetic), eGFP (Synthetic)Mammalian ExpressionA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Expresses Tandem Dimer smURFP and eGFP in mammalian cells. pcDNA3 Add to Cart
    80345pcDNA3-smURFP-IRES-HO-1smURFP (Synthetic), HO-1 (Other)Mammalian ExpressionA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Expresses smURFP and Heme Oxygenase-1 in mammalian cells. pcDNA3 Add to Cart
    80346pcDNA3-TDsmURFP-IRES-HO-1Tandem Dimer smURFP (Synthetic), HO-1 (Other)Mammalian ExpressionA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells. pcDNA3 Add to Cart
    80347pLenti-smURFP-T2A-mCherrysmURFP (Synthetic), mCherry (Synthetic)LentiviralA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry. pLenti Add to Cart
    80348pLenti-smURFP-T2A-mCardinalsmURFP (Synthetic), mCardinal (Synthetic)LentiviralA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal. pLenti Add to Cart
    80349pLenti-smURFPsmURFP (Synthetic)LentiviralA far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935. Lentiviral transfer vector under the CMV promoter that expresses smURFP. pLenti Add to Cart
    91757pNCS BFP Blue Fluorescent Protein (Other)Bacterial ExpressionUnderstanding, improving and using green fluorescent proteins. Trends Biochem Sci. 1995 Nov;20(11):448-55. Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91759pNCS YFPVenus YFP (A206K) (Other)Bacterial ExpressionUnderstanding, improving and using green fluorescent proteins. Trends Biochem Sci. 1995 Nov;20(11):448-55. Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91760pNCS mHoneydewmonomeric Honeydew (Other)Bacterial ExpressionImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew) modified pRSETB
  • Tag / Fusion Protein
    • no tags
  • Add to Cart
    91761pNCS CitrineCitrine (Other)Bacterial ExpressionReducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. Bacterial expression of a fluorescent protein, Citrine modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91763pNCS mTangerinemonomeric Tangerine (Other)Bacterial ExpressionImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine) modified pRSETB
  • Tag / Fusion Protein
    • no tag
  • Add to Cart
    91764pNCS mOrangemonomeric Orange (Other)Bacterial ExpressionImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. Bacterial expression of a fluorescent protein, monomeric Orange (mOrange) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91765pNCS mRFPmonomeric Red Fluorescent Protein1 (Other)Bacterial ExpressionA monomeric red fluorescent protein. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82. Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91767pNCS tdTomatotandem dimer Tomato (Other)Bacterial ExpressionImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91768pNCS mStrawberrymonomeric Strawberry (Other)Bacterial ExpressionImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91769pNCS mCherrymonomeric Cherry (Other)Bacterial ExpressionImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry) modified pRSETB
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91772pBAD mApplemonomeric Apple (Other)Bacterial ExpressionImproving the photostability of bright monomeric orange and red fluorescent proteins. Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. Bacterial expression of a fluorescent protein, monomeric Apple (mApple) pBAD
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
  • Add to Cart
    91773pBAD mPlummonomeric Plum (Other)Bacterial ExpressionEvolution of new nonantibody proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov 19. Bacterial expression of a fluorescent protein, monomeric Plum (mPlum) pBAD
  • Tag / Fusion Protein
    • no tag
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    91774pBAD miniSOGmini Singlet Oxygen Generator (Arabidopsis thaliana)Bacterial ExpressionA genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG) pBAD
  • Tag / Fusion Protein
    • Hexa-Histidine tag (C terminal on backbone)
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    91775pBAD IFP1.4Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (Other)Bacterial ExpressionMammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683. Bacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4) modified pBAD (+ HO-1)
  • Tag / Fusion Protein
    • Hexa-Histidine tag (C terminal on backbone)
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