Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

OCT4-eGFP-2A-Puro
(Plasmid #31939)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31939 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PCR2.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 6804
  • Vector type
    targeting donor
  • Selectable markers
    Puro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OCT4
  • Alt name
    Pou5F1
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    eGFP
  • Entrez Gene
    eGFP (a.k.a. pPRS3a_01)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OCT4-eGFP-2A-Puro was a gift from Rudolf Jaenisch (Addgene plasmid # 31939 ; http://n2t.net/addgene:31939 ; RRID:Addgene_31939)
  • For your References section:

    Genetic engineering of human pluripotent cells using TALE nucleases. Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927. 10.1038/nbt.1927 PubMed 21738127