168,234 results

1. pAAV-CAG-NLS-GFP

   Plasmid

   #104061

   Purpose

   AAV expression of nuclear localized GFP with SV40 NLS

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB

   Insert

   GFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xmts-mScarlet-I

   Plasmid

   #98818

   Purpose

   In vivo visualization of mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   mts
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

   Plasmid

   #168290

   Purpose

   CRISPR-activator Suntag-P65-HSF1 (SPH)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   dCas9, Suntag-P65-HSF1
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GRAB_NEmut (AAV9)

   Viral Prep

   #123310-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NEmut (#123310). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NEmut plasmid DNA. hSyn-driven expression of GRAB-NEmut control norepinephrine sensor (does not respond to NE). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Promoter

   Syn

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pAAVS1-P-CAG-GFP

   Plasmid

   #80491

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   CAG-GFP
   

   Use

   Donor vector (human)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB-gDA3h

   Plasmid

   #208699

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pet-15b-ultraID

   Plasmid

   #172879

   Purpose

   expresses ultraID in E. coli

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Tags

   myc

   Expression

   Bacterial

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCS-BioID2-HA

   Plasmid

   #74224

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-GFP (AAV9 trial size)

    Viral Prep

    #37825-AAV9.T

    Purpose

    Ready-to-use AAV9 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pUGI-NLS

    Plasmid

    #101091

    Purpose

    Expresses UGI in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-FR-GECO1a

    Plasmid

    #163681

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1a

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1a
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pCOLA-Gent-EM7-Erv1p-DsbC

    Plasmid

    #202485

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-RelA

    Plasmid

    #23255

    Depositor

    Warner Greene

    Article

    Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

    Insert

    p65 (RELA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE8.20-m

    Plasmid

    #136300

    Purpose

    expresses ABE8.20-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Drp1K38A

    Plasmid

    #45161

    Depositor

    Alexander van der Bliek, Richard Youle

    Article

    Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    HA (AYPYDVPDY)

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-hsRPA14 (MSW#503)

    Plasmid

    #208069

    Purpose

    Expression of GFP-tagged hsRPA3 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA3 (RPA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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