Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

An Argonaute phosphorylation cycle promotes microRNA-mediated silencing.
Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT
Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
91975MSCV-EGFPEGFP reporter cell line control (no miR-19 sites)
Loading...
91976MSCV-EGFP-Bulged-miR-19EGFP reporter cell line with eight bulged miR-19 sites
Loading...
91977LcV2-HygroLentiCRISPR v2 with Hygro cloned in place of Puro
Loading...
91978pLJM1-FH-AGO2-WTExpresses FLAG-HA-AGO2 (WT)
Loading...
91979pLJM1-FH-AGO2-5xAExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]
Loading...
91980pLJM1-EmptyEmpty backbone
Loading...
91981pLJM1-FH-AGO2-5xEExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]
Loading...
91982pLJM1-FH-AGO2-S828AExpresses FLAG-HA-AGO2 (S828A)
Loading...
91983pLJM1-FH-AGO2-D358KExpresses FLAG-HA-AGO2 (D358K)
Loading...
91984pLJM1-FH-AGO2-R438EExpresses FLAG-HA-AGO2 (R438E)
Loading...
91985pLJM1-FH-AGO2-K525EExpresses FLAG-HA-AGO2 (K525E)
Loading...
91986MSCV-Puro-FH-AGO2-WTExpresses FLAG-HA-AGO2 (WT)
Loading...
91987MSCV-Puro-FH-AGO2-S824AExpresses FLAG-HA-AGO2 (S824A)
Loading...
91988MSCV-Puro-FH-AGO2-T830AExpresses FLAG-HA-AGO2 (T830A)
Loading...
91989MSCV-Puro-FH-AGO2-S831AExpresses FLAG-HA-AGO2 (S831A)
Loading...
91990MSCV-Puro-FH-AGO2-S834AExpresses FLAG-HA-AGO2 (S834A)
Loading...
91991MSCV-Puro-FH-AGO2-S828AExpresses FLAG-HA-AGO2 (S828A)
Loading...
91992MSCV-Puro-FH-AGO2-5xAExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]
Loading...
91993MSCV-Puro-FH-AGO2-5xEExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]
Loading...
91994MSCV-Puro-FH-AGO2-S824EExpresses FLAG-HA-AGO2 (S824E)
Loading...
91995MSCV-Puro-FH-AGO2-S828EExpresses FLAG-HA-AGO2 (S828E)
Loading...
91996MSCV-Puro-FH-AGO2-T830EExpresses FLAG-HA-AGO2 (T830E)
Loading...
91997MSCV-Puro-FH-AGO2-S831EExpresses FLAG-HA-AGO2 (S831E)
Loading...
91998MSCV-Puro-FH-AGO2-S834EExpresses FLAG-HA-AGO2 (S834E)
Loading...
91999MSCV-Puro-FH-AGO2-S824E-S828EExpresses FLAG-HA-AGO2 (S824E-S828E)
Loading...
92000MSCV-Puro-FH-AGO2-S824E-S831EExpresses FLAG-HA-AGO2 (S824E-S831E)
Loading...
92001MSCV-Puro-FH-AGO2-S828E-S831EExpresses FLAG-HA-AGO2 (S828E-S831E)
Loading...
92002MSCV-Puro-FH-AGO2-S824E-S828E-S831EExpresses FLAG-HA-AGO2 (S824E-S828E-S831E)
Loading...
92003MSCV-Puro-FH-AGO2-S824A-T830AExpresses FLAG-HA-AGO2 (S824A-T830A)
Loading...
92004pGL3-BoxBExpresses firefly luciferase with BoxB sequences in 3' UTR
Loading...
92005pcDNA3.1+_Lambda-N-HA-PeptideExpresses Lambda-N-HA peptide
Loading...
92006pcDNA3.1+_FH-AGO2-WTExpresses FLAG-HA-AGO2 (WT)
Loading...
92007pcDNA3.1+_FH-AGO2-5xAExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]
Loading...
92008pcDNA3.1+_FH-AGO2-5xEExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]
Loading...
92009pcDNA3.1+_LN-HA-AGO2-WTExpresses Lambda-N-HA-AGO2 (WT)
Loading...
92010pcDNA3.1+_LN-HA-AGO2-5xAExpresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]
Loading...
92011pcDNA3.1+_LN-HA-AGO2-5xEExpresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]
Loading...
92012pcDNA3.1+_V5-ANKRD52Expresses V5-ANKRD52
Loading...
92013pCAGIG_V5-PPP6CExpresses V5-PPP6C, includes IRES-Emerald
Loading...
92014pCAGImC_V5-CSNK1A1Expresses V5-CSNK1A1, includes IRES-mCherry
Loading...
92015pCAGImC_EmptyEmpty backbone, includes IRES-mCherry
Loading...

Antibodies from Article