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Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms.

Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb RE, Webb RI, Teasdale RD, Parton RG
Dev Cell. 2015 Nov 23;35(4):513-25. doi: 10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67650mKate2-P2A-APEX2-GBPMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
67651APEX2-GBPMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).
67652nls-EGFPMammalian expression vector driving nls-EGFP.
67653BTK-APEX2-P2A-mKate2Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
67654AKT-APEX2-P2A-mKate2Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
67662mKate2-P2A-APEX2-TAPP1-PHMammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
67663mKate2-P2A-APEX2-2xFYVE_hrsMammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
67664mKate2-P2A-APEX2-OSBPMammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
67665mKate2-P2A-APEX2-ML1N*2Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
67666mKate2-P2A-APEX2-2xML1N(7Q)Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
67667Cav1-miniSOGMammalian expression vector driving dog Caveolin1-minSOG
67668Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherryZebrafish expression vector for ubiquitous expression of APEX2-GBP
67669pME-APEX2-NSGateway entry clone containing APEX2 with no stop codon
67670pME-mKate-P2A-APEX2-NSGateway entry clone containing mKate2-P2A-APEX2 with no stop codon
67671p3E-APEX2-P2A-mKate2Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2
67672p3E-GBPMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)
67673pME-BTK-PHGateway entry clone containing BTK-PH with no stop codon
67674pME-AKT-PHGateway entry clone containing AKT-PH with no stop codon
67675p3E-TAPP1-PHMultisite gateway entry clone for adding C-terminal fusions of TAPP1-PH
67676p3E-2xFYVE_hrsMultisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs
67677p3E-OSBPMultisite gateway entry clone for adding C-terminal fusions of OSBP
67678p3E-ML1N*2Multisite gateway entry clone for adding C-terminal fusions of ML1N*2
67679p3E-ML1N*2(7Q)Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)
67796ML1Nmut_in_pEGFP-C1Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP
67797ML1Nwt_in_pEGFP-C1Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP
71282HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherryZebrafish expression vector for heat-shock inducible expression of APEX2-GBP
71283pME-2PH-PLCdMultisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta
712842PH-PLCd-APEX2-P2A-mKate2Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Antibodies from Article