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Thomas Roberts Lab Plasmids


The Thomas Roberts Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
PI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Ilic N, Utermark T, Widlund HR, Roberts TM Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29.
Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM, Hahn WC Cancer Cell. 2003 May . 3(5):483-95.
DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM J Biol Chem. 2002 Mar 8. 277(10):7962-9.
Induction of p53-independent apoptosis by simian virus 40 small t antigen. Gjoerup O, Zaveri D, Roberts TM J Virol. 2001 Oct . 75(19):9142-55.
Constitutive cellular expression of PI 3-kinase is distinct from transient expression. Auger KR, Wang J, Narsimhan RP, Holcombe T, Roberts TM Biochem Biophys Res Commun. 2000 Jun 16. 272(3):822-9.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
10830pcDNA3 HA Arf1Arf1 (Bos taurus)Mammalian ExpressionDEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 8. 277(10):7962-9. pcDNA3.1 ARF1 BOS_7078 Add to Cart
10831pcDNA3 HA Arf6 DN T27NArf6 T27N (Homo sapiens)Mammalian ExpressionDEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 8. 277(10):7962-9. pcDNA3.1 ARF6 Add to Cart
10832pcDNA3 HA Arf1 ActQ71LArf1 Q71L (Homo sapiens)Mammalian ExpressionDEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 8. 277(10):7962-9. pcDNA3.1 ARF1 Add to Cart
10833pcDNA3 HA Arf1 DN T31NArf1 T31N (Homo sapiens)Mammalian ExpressionDEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 8. 277(10):7962-9. pcDNA3.1 ARF1 Add to Cart
10834pcDNA3 HA Arf6Arf6 (Homo sapiens)Mammalian ExpressionDEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 8. 277(10):7962-9. pcDNA3.1 ARF6 Add to Cart
10835pcDNA3 HA Arf6 ActQ67LArf6 Q67L (Homo sapiens)Mammalian ExpressionDEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 8. 277(10):7962-9. pcDNA3.1 ARF6 Add to Cart
10836pLNCX Myr His p110p110 (Bos taurus)Mammalian Expression, RetroviralConstitutive cellular expression of PI 3-kinase is distinct from transient expression. Biochem Biophys Res Commun. 2000 Jun 16. 272(3):822-9. pLNCX PIK3CA Add to Cart
10837pLNCX Myr His p110 KDp110 KD (Bos taurus)Mammalian Expression, RetroviralConstitutive cellular expression of PI 3-kinase is distinct from transient expression. Biochem Biophys Res Commun. 2000 Jun 16. 272(3):822-9. pLNCX PIK3CA Add to Cart
10838pcDNA3 flag p53p53 (Homo sapiens)Mammalian ExpressionInduction of p53-independent apoptosis by simian virus 40 small t antigen. J Virol. 2001 Oct . 75(19):9142-55. pcDNA3 TP53 BCC7, LFS1, P53, TRP53 Add to Cart
13970pBABE-puro SV40 LTSV40 LTMammalian Expression, RetroviralHuman mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Cancer Cell. 2003 May . 3(5):483-95. pBABE-puro SV40gp6 SV40gp6 Add to Cart
33252pBabe puro-eIF4EeIF4E (Homo sapiens)Mammalian Expression, RetroviralPI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29. pBabe puro EIF4E AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E Add to Cart
33253pBabe puro-HA-PIK3CA(H1047R;I848V)PIK3CA (Homo sapiens)Mammalian Expression, RetroviralPI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29. pBabe puro PIK3CA CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha Add to Cart
33254pBabe puro-HA-PIK3CA(H1047R;G320E)PIK3CA (Homo sapiens)Mammalian Expression, RetroviralPI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29. pBabe puro
  • Tag / Fusion Protein
    • Hemagglutinin (HA) (N terminal on insert)
  • PIK3CA CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
    Add to Cart
    33255pBabe puro-HA-PIK3CA(H1047R;F486S;del)PIK3CA (Homo sapiens)Mammalian Expression, RetroviralPI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29. pBabe puro
  • Tag / Fusion Protein
    • Hemagglutinin (HA) (N terminal on insert)
  • PIK3CA CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
    Add to Cart