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pTriEx4-Rac1-2G
(Plasmid #66110)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66110 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 66110-D50 50 μg of DNA in Tris buffer $495
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DNA 66110-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTriEx4
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 7518
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Second generation Rac1 FRET biosensor
  • Alt name
    Rac1-2G
  • Species
    Synthetic
  • Insert Size (bp)
    2576
  • Promoter CMV
  • Tag / Fusion Protein
    • His-Myc (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ctggttattgtgctgtctc
  • 3′ sequencing primer tcagtggtatttgtga
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTriEx4-Rac1-2G was a gift from Olivier Pertz (Addgene plasmid # 66110 ; http://n2t.net/addgene:66110 ; RRID:Addgene_66110)

  • For your References section:

    SrGAP2-Dependent Integration of Membrane Geometry and Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of Locomotion. Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O. Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 10.1016/j.devcel.2015.09.002. 10.1016/j.devcel.2015.09.002 PubMed 26439400
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