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Plasmid#198756PurposeVector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418DepositorInsertTet3G, IRES, Neo
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:Ascl1
Plasmid#127289Purposeall-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)DepositorUseLentiviralTagsNgn2 and Ascl1 linked via 2AExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX puro TRIM21-GFP
Plasmid#116941PurposeExpression of TRIM21-GFP fusion proteinDepositorInsertTRIM21 - GFP (TRIM21 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR2-V5/HIS
Plasmid#205617PurposeExpression of human VEGFR2 receptor tyrosine kinase in mammalian cellsDepositorInsertVEGFR2 receptor tyrosine kinase (KDR Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pcDNA3-CKAR2
Plasmid#123348PurposeImproved FRET-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCKAR2
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-PMr
Plasmid#61180PurposeFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPIP2a
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralTagsExpressionMutationDR1 A>TPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4) (Lentiviral Prep)
Viral Prep#17446-LVPurposeReady-to-use Lentiviral Prep particles produced from pLenti CMV GFP Hygro (656-4) (#17446). In addition to the viral particles, you will also receive purified pLenti CMV GFP Hygro (656-4) plasmid DNA. Lentiviral particles carrying the GFP and hygromycin resistance.DepositorPromoterTagsNoneAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucOS
Plasmid#22777DepositorInsertOVA-SIY
UseCre/Lox and LentiviralTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
mScarletI-Rab7
Plasmid#112955PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorArticleInsertRab7
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML104
Plasmid#67638PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52 and pTDH3Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRmine-mScarlet-WPRE
Plasmid#130994PurposeAAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoterDepositorInsertChRmine
UseAAVTagsmScarletExpressionMutationPromoterhSynAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE p38alpha
Plasmid#82719PurposeExpresses constitutively active human MKK6 and murine p38 alpha.DepositorUseTagsExpressionBacterialMutationSer207 to Glu, Thr211 to GluPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEM415-ldhL-mRFP1
Plasmid#99842PurposeExpression of RFP in LactobacillusDepositorInsertmonomeric red fluorescent protein
UseTagsFLAGExpressionBacterialMutationPromoterldhlAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM4D(Gi)-mCherry (AAV5)
Viral Prep#50479-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-GFAP-hM4D(Gi)-mCherry (#50479). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM4D(Gi)-mCherry plasmid DNA. GFAP-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsmCherryAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only