168,037 results

1. pLEX_305-C-dTAG

   Plasmid

   #91798

   Purpose

   Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rich-pT7

   Plasmid

   #108445

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Promoter

   pT7

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-tadDE-PcrA M6-UGI

   Plasmid

   #221236

   Purpose

   Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   tadDE-PcrA M6-UGI
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-FLEX-EGFP-WPRE

   Plasmid

   #51502

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-miR-142

   Plasmid

   #117056

   Purpose

   Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

   Depositor

   Michele De Palma

   Article

   Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

   Insert

   miR-142-3p - OFP (Mir142 Mouse)
   

   Use

   Lentiviral

   Promoter

   SFFV promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mfn2-Myc

   Plasmid

   #23213

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn2 (Mfn2 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. SNX9-pmCherryC1

   Plasmid

   #27678

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   SNX9 (Snx9 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-mito-ApplePy1Highest

    Plasmid

    #239074

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Highest
    

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP-hCOL1A1

    Plasmid

    #140110

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag, K20…

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-mBATF

    Plasmid

    #34575

    Depositor

    Peter Murray

    Article

    Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

    Insert

    BATF (Batf Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pHEN2-SG4

    Plasmid

    #196071

    Purpose

    Express wild type SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4
    

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Expression

    Mammalian

    Mutation

    H840A

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

    Plasmid

    #232800

    Purpose

    Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    OsTIR1 F74G EGFP-AID
    

    Use

    Lentiviral

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-EGFP (AAV5)

    Viral Prep

    #50457-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

18. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMG-RIS

    Plasmid

    #225132

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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