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mScarlet: a bright monomeric red fluorescent protein for cellular imaging.
Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85042pmScarlet_C1Mammalian expression of mScarlet
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85043pmScarlet-H_C1Mammalian expression of mScarlet-H
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85044pmScarlet-i_C1Mammalian expression of mScarlet-i
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85045pmScarlet_alphaTubulin_C1In vivo visualization of microtubules (can be used for colocalization studies)
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85046pmScarlet-H_alphaTubulin_C1In vivo visualization of microtubules (can be used for colocalization studies)
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85047pmScarlet-i_alphaTubulin_C1In vivo visualization of microtubules (can be used for colocalization studies)
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85048pmScarlet_Giantin_C1In vivo visualization of the Golgi apparatus (can be used for colocalization studies)
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85049pmScarlet-H_Giantin_C1In vivo visualization of the Golgi apparatus (can be used for colocalization studies)
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85050pmScarlet-i_Giantin_C1In vivo visualization of the Golgi apparatus (can be used for colocalization studies)
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85051pmScarlet_H2A_C1In vivo visualization of the nucleus (can be used for colocalization studies)
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85052pmScarlet-H_H2A_C1In vivo visualization of the nucleus (can be used for colocalization studies)
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85053pmScarlet-i_H2A_C1In vivo visualization of the nucleus (can be used for colocalization studies)
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85054pLifeAct_mScarlet_N1In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
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85055pLifeAct_mScarlet-H_N1In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
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85056pLifeAct_mScarlet-i_N1In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
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85057pMTS_mScarlet_N1In vivo visualization of the mitochondria (can be used for colocalization studies)
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85058pMTS_mScarlet-H_N1In vivo visualization of the mitochondria (can be used for colocalization studies)
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85059pMTS_mScarlet-i_N1In vivo visualization of the mitochondria (can be used for colocalization studies)
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85060pmScarlet_NES_C1In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)
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85061pmScarlet-H_NES_C1In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)
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85062pmScarlet-i_NES_C1In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)
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85063pmScarlet_peroxisome_C1In vivo visualization of the peroxisome (can be used for colocalization studies)
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85064pmScarlet-H_peroxisome_C1In vivo visualization of the peroxisome (can be used for colocalization studies)
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85065pmScarlet-I_peroxisome_C1In vivo visualization of the peroxisome (can be used for colocalization studies)
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85066pCytERM_mScarlet_N1In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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85067pCytERM_mScarlet-H_N1In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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85068pCytERM_mScarlet-i_N1In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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85069PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)
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85070PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)
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85071pTriEx-RhoA-wt_mScarlet-i_SGFP2Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity
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85072pTriEx-RhoA-Q63L_mScarlet-i_SGFP2Green/red FRET biosensor for RhoA in the GTP locked constitutively active state
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110623PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.
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