PurposeMammalian expression of mScarlet-i
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85044||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||85044-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)701
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 85044-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmScarlet-i_C1 was a gift from Dorus Gadella (Addgene plasmid # 85044 ; http://n2t.net/addgene:85044 ; RRID:Addgene_85044)
For your References section:mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. 10.1038/nmeth.4074 PubMed 27869816