163,819 results

1. pX330_SYCP3_nterm

   Plasmid

   #222524

   Purpose

   Cas9/sgRNA plasmid for targeting SYCP3

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   Cas9, SYCP3 sgRNA (SYCP3 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiPGK Puro DEST JNKKTRrsGreenF-E

   Plasmid

   #205764

   Purpose

   Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   JNK KTR rsGreenF-E
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCMV Puro DEST ERKKTRrsFastLime

   Plasmid

   #205765

   Purpose

   Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   ERK KTR rsFastLime
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERH-STRA8

   Plasmid

   #222585

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   STRA8 (STRA8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lck-Halo_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221900

   Purpose

   Expresses Halo7 targeted to the plasma membrane

   Depositor

   Janelia Integrative Imaging

   Insert

   Lck-Halo7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. RoL10X_pET29b

   Plasmid

   #215427

   Purpose

   Expression construct for RoL10X gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   RoL10X
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ThcCut1_pET29b

   Plasmid

   #215429

   Purpose

   Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   ThcCut1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR182-DuET

   Plasmid

   #213272

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR182 (GPR182 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPLICSFAST-ER-PM-P2A

   Plasmid

   #214893

   Purpose

   Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PM-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid

    #211760

    Purpose

    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

    Depositor

    Glenn Yiu

    Article

    Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0751

    Plasmid

    #143729

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIVEP1 (HIVEP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-AM

    Plasmid

    #206513

    Purpose

    AAV packaging vector containing AAV2 rep and AM cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Gonzalez-Sandoval et al Nat Commun. 2023 Apr 28;14(1):2448. doi:

    Insert

    AM capsid
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p5

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-Ruby2-NS

    Plasmid

    #166569

    Purpose

    Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Ruby2
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. acta1-mRuby2-VHH[GFP]

    Plasmid

    #163856

    Purpose

    Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    VHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-Cavin4b-TurboID

    Plasmid

    #163851

    Purpose

    In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3TS-CD44b-TurboID

    Plasmid

    #163850

    Purpose

    In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    CD44b (cd44b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS-BASU-EGFP

    Plasmid

    #163845

    Purpose

    In vitro transcription of BASU-EGFP. Parton lab clone JRR

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    BASU
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2plus-TDP-43 M337V

    Plasmid

    #162609

    Purpose

    Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M337V

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGMC00005

    Plasmid

    #166722

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMC00006

    Plasmid

    #166723

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge