247,492 results

1. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

   Plasmid

   #187576

   Purpose

   Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-hChR2(H134R)-EYFP (AAV1)

   Viral Prep

   #26973-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   EYFP

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV2)

   Viral Prep

   #100836-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   CAG

   Tags

   None

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

4. Mouse Validation (mVAL) CRISPR Library

   Pooled Library

   #159391

   Purpose

   Pooled CRISPR library targets core intrinsic immune evasion genes

   Depositor

   Jason Moffat

   Article

   Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR and Lentiviral

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-sapphireCKAR

   Plasmid

   #118468

   Purpose

   cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDuet TDP-43 Q331K

   Plasmid

   #27465

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Tags

   Expression

   Bacterial

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGW_GPHN.FingR-YFP

   Plasmid

   #46298

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR (GPHN Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMU-GAr

   Plasmid

   #61172

   Purpose

   Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   GmMAN49-mCherry
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.EZH2

   Plasmid

   #125161

   Purpose

   Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Baculovirus expression vector

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-hTDG

    Plasmid

    #70758

    Purpose

    bacterial expression of human TDG

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    TDG (TDG Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV1)

    Viral Prep

    #130998-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    EF1a

    Tags

    mScarlet

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmr101A-hVPS26

    Plasmid

    #17636

    Depositor

    James Hurley

    Article

    Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

    Insert

    VPS26 (VPS26A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hsyn-GRAB_Ado1.0

    Plasmid

    #153285

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV GFP1-9

    Plasmid

    #182244

    Purpose

    Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP 1-9 OPT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF.hICN1.Ubc.GFP

    Plasmid

    #17626

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCruzHA SIRT1

    Plasmid

    #10962

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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