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HLTV-hTRIM21
(Plasmid #104973)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104973 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HLTV
  • Backbone manufacturer
    Mark Allen (MRC LMB)
  • Backbone size w/o insert (bp) 3113
  • Total vector size (bp) 4513
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tripartite Motif containing 21
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • GenBank ID
  • Entrez Gene
    TRIM21 (a.k.a. RNF81, RO52, Ro/SSA, SSA, SSA1)
  • Promoter T7
  • Tag / Fusion Protein
    • His-Lipoyl (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HLTV-hTRIM21 was a gift from Leo James (Addgene plasmid # 104973 ; http://n2t.net/addgene:104973 ; RRID:Addgene_104973)
  • For your References section:

    A Method for the Acute and Rapid Degradation of Endogenous Proteins. Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M. Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 10.1016/j.cell.2017.10.033. 10.1016/j.cell.2017.10.033 PubMed 29153837