163,882 results

1. pCAG-EGFP-C-IFT88

   Plasmid

   #218733

   Purpose

   Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

   Insert

   IFT88 (IFT88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPM2213

   Plasmid

   #167553

   Purpose

   Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

   Depositor

   Renato Morona

   Article

   Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

   Insert

   rfb region of Shigella flexneri serotype 2a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP NFAT5 (no EGFP)

   Plasmid

   #13627

   Depositor

   Anjana Rao

   Article

   Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSTB_pCSdest

   Plasmid

   #53910

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   CSTB (CSTB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pAAV_GR-RE_MLPmin_BC1092-luc2

   Plasmid

   #227112

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMX-Brg1

   Plasmid

   #25855

   Depositor

   Hans Schöler

   Article

   Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

   Insert

   Brahma related gene 1 (Smarca4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-VTKS2-TVA-eGFP-N2cG

   Plasmid

   #175439

   Purpose

   Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

   Depositor

   Gordon Fishell

   Article

   Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

   Insert

   TVA-P2A-eGFP-P2A-N2cG
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV CAMKII PSAM4 GlyR IRES EGFP

   Plasmid

   #119744

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CamKII

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-EB3mCherry_cmv-LifeActGFP

   Plasmid

   #133927

   Purpose

   Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-Rab7A

    Plasmid

    #131417

    Purpose

    Expresses HA-tagged human Rab7A in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    human Rab7A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCACTUS

    Plasmid

    #167559

    Purpose

    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

    Type

    Empty backbone

    Use

    Suicide cloning vector, temperature sensitive.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLOX-CW-CRE

    Plasmid

    #12238

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    CRE
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

    Plasmid

    #113163

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Hyg-mEGFP

    Plasmid

    #191847

    Purpose

    Expresses the monomeric form of EGFP

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Flag mTOR wt

    Plasmid

    #26603

    Purpose

    Mammalian expression of human flag-tagged mTor

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. LCV2 control

    Plasmid

    #217443

    Purpose

    Lentiviral vector expressing Cas9 without a targeting sgRNA

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2541

    Plasmid

    #142399

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX5 (SOX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW036

    Plasmid

    #140034

    Purpose

    KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cpt

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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