163,852 results

1. pRK5-hGRK4

   Plasmid

   #32690

   Depositor

   Robert Lefkowitz

   Article

   Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

   Insert

   GRK4 (GRK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221_CLN3

   Plasmid

   #131868

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   CLN3 (CLN3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pT7C-DNAJA1

   Plasmid

   #177655

   Purpose

   Recombinant protein synthesis of human DNAJA1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   DNAJA1 (DNAJA1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GPR87-Tango

   Plasmid

   #66379

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR87 (GPR87 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV9)

   Viral Prep

   #162382-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Tags

   None

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

6. CP1041-ABEmax

   Plasmid

   #119808

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-TurboID-NS

   Plasmid

   #166566

   Purpose

   Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF0214

   Plasmid

   #142486

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXP1 (FOXP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pStr-KDEL_TPV-CXCL9-mCherry-SBP

   Plasmid

   #170677

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   CXCL9 (CXCL9 Human, Synthetic)
   streptavidin-KDEL
   

   Use

   Tags

   SBP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and T7

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SrtA-PDGFR Tm

    Plasmid

    #121168

    Purpose

    pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

    Depositor

    Gabriel Victora

    Article

    Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

    Insert

    SrtA-PDGFR
    

    Use

    Retroviral

    Tags

    Tomato and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. bRA66

    Plasmid

    #100952

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lentiviral-TOP-dGFP-reporter

    Plasmid

    #14715

    Purpose

    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    LEF-1/TCF-responsive promoter (Ctnnb1 )
    

    Use

    Lentiviral

    Tags

    d2 eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE GFP-Grin1 KI #3

    Plasmid

    #139659

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK1008 (pSL7)

    Plasmid

    #191003

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2408

    Plasmid

    #143370

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXF1 (FOXF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe neo IRES-EGFP

    Plasmid

    #14431

    Depositor

    L. Miguel Martins

    Insert

    IRES-EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. STAGR_gRNAScaffold_mU6

    Plasmid

    #102844

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert gRNAScaffold_mU6
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO GFP DNAJB9

    Plasmid

    #19505

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB9 (DNAJB9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Human SHIP1 (clone hp51CN)

    Plasmid

    #123347

    Purpose

    Expression of human SHIP1

    Depositor

    Christophe Erneux

    Article

    Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

    Insert

    INPP5D (INPP5D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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