161,601 results

1. pCG-Hc∆18

   Plasmid

   #84817

   Purpose

   Measles virus hemagglutinin expressing plasmid

   Depositor

   Jakob Reiser

   Article

   Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

   Insert

   Measles virus H glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

   Plasmid

   #182925

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Coff/Fon-GCaMP6M (AAV8)

   Viral Prep

   #137121-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6M (#137121). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6M plasmid DNA. EF1a-driven expression of GCaMP6m in the presence of Flp recombinase (inhibited in the presence of Cre recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   None

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pHEN2-SG4

   Plasmid

   #196071

   Purpose

   Express wild type SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4
   

   Use

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-VSVL

   Plasmid

   #64085

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

   Depositor

   Ian Wickersham

   Insert

   VSV polymerase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)

   Viral Prep

   #51085-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting

   Promoter

   Syn

   Tags

   nuclear dTomato (physically separate, not a fusion protein)

   Available since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pLJC6-3XHA-TMEM192

   Plasmid

   #104434

   Purpose

   Expresses 3XHA-TMEM192 in mammalian cells

   Depositor

   David Sabatini

   Article

   Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mcherry-DN KASH

   Plasmid

   #125553

   Purpose

   mcherry labeled DN-KASH

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Truncated form: only the KASH domain of nesprin-…

   Promoter

   CMV

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBRPB CAG-mCherry-IP

   Plasmid

   #106333

   Purpose

   PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   mCherry-IRES-Puro
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-SYK

    Plasmid

    #23907

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SYK (SYK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVT-iCre

    Plasmid

    #232773

    Purpose

    IVT template for making modRNA encoding iCre

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    iCre
    

    Use

    Cre/Lox; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1-GT_AIO-TetOn_Divergent Donor

    Plasmid

    #229784

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GA_AIO-TetOn_Divergent Donor

    Plasmid

    #229786

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid

    #223113

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.GFAP.eGFP.WPRE.hGH (AAV5)

    Viral Prep

    #105549-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.GFAP.eGFP.WPRE.hGH (#105549). In addition to the viral particles, you will also receive purified pAAV.GFAP.eGFP.WPRE.hGH plasmid DNA. GFAP-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    GFAP

    Tags

    EGFP

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB301-2μ-HDV

    Plasmid

    #196329

    Purpose

    A yeast-Escherichia coli-Agrobacterium shuttle vector

    Depositor

    Zhenghe Li

    Article

    Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNCS mRFP

    Plasmid

    #91765

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Insert

    monomeric Red Fluorescent Protein1
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CaV2.1/pcDNA3.1 ~ Human

    Plasmid

    #140575

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    CaV2.1 Human (CACNA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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