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pCMV5 TrkA
(Plasmid #15002)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15002 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TrkA
  • Alt name
    Trk A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    V289L
  • Entrez Gene
    NTRK1 (a.k.a. MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TrkA was originally from B Hempstead, and was generated in the lab of Dr. Moses Chao.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The mammalian expression plasmid for trkA was generated by subcloning of TrkA full-length cDNA into pCMV5 vector at the EcoRI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 TrkA was a gift from Raymond Birge (Addgene plasmid # 15002 ; http://n2t.net/addgene:15002 ; RRID:Addgene_15002)
  • For your References section:

    Association of the Abl tyrosine kinase with the Trk nerve growth factor receptor. Yano H, Cong F, Birge RB, Goff SP, Chao MV. J Neurosci Res. 2000 Feb 1. 59(3):356-64. 10.1002/(SICI)1097-4547(20000201)59:3<356::AID-JNR9>3.0.CO;2-G PubMed 10679771