|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15002||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameTrk A
SpeciesH. sapiens (human)
Insert Size (bp)2400
Entrez GeneNTRK1 (a.k.a. MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
The mammalian expression plasmid for trkA was generated by subcloning of TrkA full-length cDNA into pCMV5 vector at the EcoRI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 TrkA was a gift from Raymond Birge (Addgene plasmid # 15002 ; http://n2t.net/addgene:15002 ; RRID:Addgene_15002)
For your References section:Association of the Abl tyrosine kinase with the Trk nerve growth factor receptor. Yano H, Cong F, Birge RB, Goff SP, Chao MV. J Neurosci Res. 2000 Feb 1. 59(3):356-64. 10.1002/(SICI)1097-4547(20000201)59:3<356::AID-JNR9>3.0.CO;2-G PubMed 10679771