Approaching infinite affinity through engineering of peptide-protein interaction.
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytonen VP, Howarth M
Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 10.1073/pnas.1909653116. PubMed Article
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytonen VP, Howarth M
Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 10.1073/pnas.1909653116. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133447 | SpyCatcher003 | Expresses SpyCatcher003 in bacterial cytoplasm |
| 133448 | SpyCatcher003 S49C | Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling |
| 133449 | SpyCatcher003-sfGFP | Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm |
| 133450 | SpyTag003-MBP | Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm |
| 133451 | TfR-sfGFP-myc tag-SpyCatcher003 | Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection |
| 133452 | SpyTag003-mKate2 | Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm |
| 133453 | SpyTag003-mClover3 | Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm |
| 133454 | SpyTag003-sfGFP | Expresses SpyTag003-superfolder GFP in bacterial cytoplasm |
| 133566 | EGFP-Talin head-SpyCatcher003 | Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells |
| 133567 | SpyTag003-Talin rod-mCherry | Expresses SpyTag003-Talin rod-mCherry in mammalian cells |