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Approaching infinite affinity through engineering of peptide-protein interaction.
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytonen VP, Howarth M
Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 10.1073/pnas.1909653116.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
133447SpyCatcher003Expresses SpyCatcher003 in bacterial cytoplasm
133448SpyCatcher003 S49CExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling
133449SpyCatcher003-sfGFPExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasm
133450SpyTag003-MBPExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm
133451TfR-sfGFP-myc tag-SpyCatcher003Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
133452SpyTag003-mKate2Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm
133453SpyTag003-mClover3Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm
133454SpyTag003-sfGFPExpresses SpyTag003-superfolder GFP in bacterial cytoplasm
133566EGFP-Talin head-SpyCatcher003Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells
133567SpyTag003-Talin rod-mCherryExpresses SpyTag003-Talin rod-mCherry in mammalian cells

Antibodies from Article