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159,843 results

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pEHAC1-HA-BirA-GSGS-Traf6

Plasmid

#232586

Purpose

Express BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA -TRAF6 (TRAF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub-dGG

Plasmid

#232581

Purpose

Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP(-3)-Ub

Plasmid

#232583

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Use

Tags

AP

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub

Plasmid

#232577

Purpose

Express Ubiquitin tagged with an Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-GSGS-BirA-HA

Plasmid

#232588

Purpose

Express BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-FOXA1

Plasmid

#232645

Purpose

transient expresses FOXA1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSEED-Dendra2

Plasmid

#219645

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Dendra2 SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221075

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Tags

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTA-HUH

Plasmid

#221152

Purpose

HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Insert

HisG-URA3-HisG

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pOCC120-MBP-DDX3X-monoGFP-His

Plasmid

#219985

Purpose

DDX3X(WT) protein purification

Depositor

Simon Alberti

Insert

DDX3X(WT) (DDX3X Human)

Use

Tags

MBP and monoGFP and 6xHis

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_836

Plasmid

#216088

Purpose

Cas9 [Sp] knockout targeting CD55, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD55 guide

Use

CRISPR and Lentiviral; Positive controls

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_GTOV_GPC

Plasmid

#207277

Purpose

Mammalian expression of Guanarito virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

GTOV_GPC

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-mCh-P2A-ST-EGFP-CAAX

Plasmid

#207636

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Amber stop codon at SpyCatcher’s critical lysine

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-moxBFP-WPRE

Plasmid

#194976

Purpose

AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1053A

Plasmid

#184006

Purpose

Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNAeve1 - U6c:sgRNAeve2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CIP2Aprom108bp-pGL4.10Luc

Plasmid

#60875

Purpose

Luc reporter driven by 108 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

NM_020890108 bp promoter fragment of Cancerous Inhibitor of PP2A.2 (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pCDH-CMV-mCherry-T2A-Puro

Plasmid

#72264

Purpose

Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

Depositor

Kazuhiro Oka

Insert

mCherry-T2A-Puro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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