165,839 results

1. pLp_3050sNuc

   Plasmid

   #122030

   Purpose

   Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Vincent Eijsink

   Article

   Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

   Insert

   signal peptide Lp_3050
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIRV-AP-1-mCherry

   Plasmid

   #118095

   Purpose

   Fluorescent reporter for AP-1 activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with AP-1 responsive element

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS130

   Plasmid

   #26290

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFD116

   Plasmid

   #124769

   Purpose

   pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   dcas9
   PpflB sgRNA BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p206-Switch-ON

   Plasmid

   #217885

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRH-1363 LV EF1a-CD19 IRES-EGFP

   Plasmid

   #201919

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIVTRup

    Plasmid

    #101362

    Purpose

    Highly efficient in vitro transcription. Empty backbone.

    Depositor

    Ángel Raya

    Insert

    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    

    Use

    For cloning a gene for in vitro transcription pur…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

12. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. AdenoBuilder toolkit

    Plasmid Kit

    #1000000176

    Purpose

    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.

    Depositor

    Fred Bunz

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. FHRE-Luc

    Plasmid

    #1789

    Purpose

    Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FHRE (FOXO3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    3 copies of FRE

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CamKIIa-jGCaMP8m-WPRE (AAV9)

    Viral Prep

    #176751-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CamKIIalpha

    Tags

    None

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

18. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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