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Farren Isaacs Lab Plasmids


The Farren Isaacs Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
CAGE (unpublished)
Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll D, Rinehart J, Isaacs FJ Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51946rEc2 CAGE (unpublished) oriT-kan template NA Add to Cart
51948rEc 27.H.Sc CAGE (unpublished) specR template NA Add to Cart
51950pRK24E. coli Conjugation genes CAGE (unpublished) broad-host range natural conjugative plasmid RK24 Add to Cart
73544pEvol-pAcFRS.2.t1pAcFRS.2.t1 (Synthetic), pAcFRS.2.t1 (Synthetic)Bacterial ExpressionEvolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16. tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG p15A Add to Cart
73545pEvol-pAcFRS.1.t1pAcFRS.1.t1 (Synthetic), pAcFRS.1.t1 (Synthetic)Bacterial ExpressionEvolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16. tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG p15A Add to Cart
73546pEvol-pAzFRS.2.t1pAzFRS.2.t1 (Synthetic), pAzFRS.2.t1 (Synthetic)Bacterial ExpressionEvolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16. tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG p15A Add to Cart
73547pEvol-pAzFRS.1.t1pAzFRS.1.t1 (Synthetic), pAzFRS.1.t1Bacterial ExpressionEvolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16. tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG p15A Add to Cart
73581C321.deltaA (Isaacs lab)noneBacterial ExpressionEvolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16. Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene NA Add to Cart