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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Publication

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Publication

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Publication

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Publication

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Publication

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Publication

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Publication

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Publication

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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MIGR1

Plasmid

#27490

Depositor

Warren Pear

Publication

Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

93 Articles

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MIGR1

Plasmid

#27490

Depositor

Warren Pear

Publication

Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

93 Articles

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pEZYmyc-His

Plasmid

#18701

Purpose

pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Publication

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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