165,839 results

1. pET24b-APEX2

   Plasmid

   #111702

   Purpose

   For IPTG-inducible expression of APEX2 in E. coli

   Depositor

   Jessica Seeliger

   Article

   Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

   Insert

   APEX2
   

   Use

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_3XHA-ATP6V1B2-mNeonGreen

   Plasmid

   #228865

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mNeonGreen

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9-mCherry-Frame +2

   Plasmid

   #66941

   Purpose

   CRISPaint frame selector +2

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFD116

   Plasmid

   #124769

   Purpose

   pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   dcas9
   PpflB sgRNA BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV.CBA.eGFP.2A.P301L-Tau

   Plasmid

   #140425

   Purpose

   AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

   Depositor

   Bradley Hyman

   Article

   Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

   Insert

   eGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   changed Proline 301 to Leucine

   Promoter

   CBA

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHTN_HaloTag_KIF5B

   Plasmid

   #213404

   Purpose

   Full-length human kinesin-1 with HaloTag at the N-terminus

   Depositor

   Jonas Ries

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   HaloTag-KIF5B (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-MCS1-2A-MCS2

   Plasmid

   #71782

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHW91013

   Plasmid

   #222450

   Purpose

   Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-miniDFR::RUBY::OCS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule2-pCNF

    Plasmid

    #85495

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Promoter

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-fDIO-GCaMP6s (AAV8)

    Viral Prep

    #105714-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO-GCaMP6s (#105714). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6s plasmid DNA. EF1a-driven, Flp recombinase-dependent expression of GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals

    Depositor

    Rylan Larsen

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Tags

    None

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pBMN(CMV-copGFP-Puro-SMAR)

    Plasmid

    #80391

    Purpose

    expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Puromycine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV Neo DEST (705-1)

    Plasmid

    #17392

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV PHP.eB)

    Viral Prep

    #44362-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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