Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

169,450 results

Showing: 3181 - 3200 of 169450 results

  1. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Promoter
    Cbh
    Available Since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74630-plasmid-map-sequence-id-373032

    Enlarge

  2. TFORF1479

    Plasmid
    #143504
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143504-plasmid-map-sequence-id-400403

    Enlarge

  4. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

    Enlarge

  9. TFORF1481

    Plasmid
    #141827
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141827-plasmid-map-sequence-id-391425

    Enlarge

  13. pcDNA3.1-N-Luc

    Plasmid
    #185357
    Purpose
    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185357-plasmid-map-sequence-id-363972

    Enlarge

  14. enOgeuIscB with ωRNA-v2

    Plasmid
    #220939
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220939-plasmid-map-sequence-id-443121

    Enlarge

  16. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep
    #98747-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

    Enlarge

Showing: 3181 - 3200 of 169450 results