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Depositing Lab
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Depositing OrganizationUniversity of Texas MD Anderson Cancer Center
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12133 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 12133-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 12133-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1A Myc-His
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTSC1
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SpeciesH. sapiens (human)
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Insert Size (bp)3500
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Entrez GeneTSC1 (a.k.a. LAM, TSC)
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Tags
/ Fusion Proteins
- myc (C terminal on backbone)
- his (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJeff DeClue
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results found a deletion of S1043 when compared to TSC1 reference sequences such as GenBank NP_001155898.1 (isoform 3) and NP_001155899.1 (isoform 4).
DNA (Catalog # 12133-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 12133-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 myc TSC1 was a gift from Cheryl Walker (Addgene plasmid # 12133 ; http://n2t.net/addgene:12133 ; RRID:Addgene_12133) -
For your References section:
Activity of TSC2 is inhibited by AKT-mediated phosphorylation and membrane partitioning. Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson CL, Kiguchi K, Walker CL. J Cell Biol. 2006 Apr 24. 173(2):279-89. 10.1083/jcb.200507119 PubMed 16636147
Map uploaded by the depositor.
