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pcDNA3.1 myc TSC1
(Plasmid #12133)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12133 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1A Myc-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Entrez Gene
    TSC1 (a.k.a. LAM, TSC)
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • his (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jeff DeClue
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results found a deletion of S1043 when compared to TSC1 reference sequences such as GenBank NP_001155898.1 (isoform 3) and NP_001155899.1 (isoform 4).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 myc TSC1 was a gift from Cheryl Walker (Addgene plasmid # 12133 ; http://n2t.net/addgene:12133 ; RRID:Addgene_12133)
  • For your References section:

    Activity of TSC2 is inhibited by AKT-mediated phosphorylation and membrane partitioning. Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson CL, Kiguchi K, Walker CL. J Cell Biol. 2006 Apr 24. 173(2):279-89. 10.1083/jcb.200507119 PubMed 16636147