|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14033||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10410
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
MutationMammalian optimized CyPet
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
The assembled sequence for this plasmid is inaccurate. The plasmid is actually 2.3kb smaller than the sequence suggests.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCEP4CyPet-MAMM was a gift from Patrick Daugherty (Addgene plasmid # 14033 ; http://n2t.net/addgene:14033 ; RRID:Addgene_14033)
For your References section:Evolutionary optimization of fluorescent proteins for intracellular FRET. Nguyen AW, Daugherty PS. Nat Biotechnol. 2005 Mar . 23(3):355-60. 10.1038/nbt1066 PubMed 15696158