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Addgene

pET-45b(+)-Tn5
(Plasmid #112112)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112112 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-45b(+)
  • Backbone size w/o insert (bp) 5260
  • Total vector size (bp) 6703
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tn5
  • Insert Size (bp)
    1443
  • Mutation
    E54K E110K P242A L372P
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GenScript
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-45b(+)-Tn5 was a gift from Frank Pugh (Addgene plasmid # 112112 ; http://n2t.net/addgene:112112 ; RRID:Addgene_112112)
  • For your References section:

    Simplified ChIP-exo assays. Rossi MJ, Lai WKM, Pugh BF. Nat Commun. 2018 Jul 20;9(1):2842. doi: 10.1038/s41467-018-05265-7. 10.1038/s41467-018-05265-7 PubMed 30030442