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(Plasmid #123461)


Item Catalog # Description Quantity Price (USD)
Plasmid 123461 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Ulrich Laemmli
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    For plasmid amplification, as well as for expression for purification, it is recommended to use the strain JM101.
  • Copy number


  • Gene/Insert name
    Protein A-Protein G-Micrococcal Nuclease
  • Alt name
  • Alt name
  • Alt name
  • Insert Size (bp)
  • Promoter Lac
  • Tags / Fusion Proteins
    • 6xHIS (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for BioRxiv preprint

This plasmid has been found to be somewhat unstable and prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. See our article on Plasmid Dimers and Multimers for more information (

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG/MNase was a gift from Steven Henikoff (Addgene plasmid # 123461 ; ; RRID:Addgene_123461)
  • For your References section:

    Improved CUT&RUN chromatin profiling tools. Meers MP, Bryson TD, Henikoff JG, Henikoff S. Elife. 2019 Jun 24;8. pii: 46314. doi: 10.7554/eLife.46314. 10.7554/eLife.46314 PubMed 31232687