PurposeExpresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123461||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerUlrich Laemmli
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsFor plasmid amplification, as well as for expression for purification, it is recommended to use the strain JM101.
Gene/Insert nameProtein A-Protein G-Micrococcal Nuclease
Insert Size (bp)1218
- Promoter Lac
/ Fusion Proteins
- 6xHIS (C terminal on insert)
- HA (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/569129v1 for BioRxiv preprint
This plasmid has been found to be somewhat unstable and prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. See our article on Plasmid Dimers and Multimers for more information (https://blog.addgene.org/plasmids-101-dimers-and-multimers).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG/MNase was a gift from Steven Henikoff (Addgene plasmid # 123461 ; http://n2t.net/addgene:123461 ; RRID:Addgene_123461)
For your References section:Improved CUT&RUN chromatin profiling tools. Meers MP, Bryson TD, Henikoff JG, Henikoff S. Elife. 2019 Jun 24;8. pii: 46314. doi: 10.7554/eLife.46314. 10.7554/eLife.46314 PubMed 31232687