PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121137||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8028
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert nameProtein A-Tnp
Insert Size (bp)2388
- Promoter T7
/ Fusion Protein
- his (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter primer
- 3′ sequencing primer T7 terminator primer (Common Sequencing Primers)
There is a single bp deletion in LacI that changes the C terminal coding sequence to FPDWKAGSERNAINVS. This change does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET15bpATnp was a gift from Keji Zhao (Addgene plasmid # 121137 ; http://n2t.net/addgene:121137 ; RRID:Addgene_121137)