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pET15bpATnp
(Plasmid #121137)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 121137 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Total vector size (bp) 8028
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein A-Tnp
  • Alt name
    His6pA-Tnp
  • Species
    E. coli
  • Insert Size (bp)
    2388
  • Promoter T7
  • Tag / Fusion Protein
    • his (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a single bp deletion in LacI that changes the C terminal coding sequence to FPDWKAGSERNAINVS. This change does not affect function.

Please visit https://doi.org/10.1101/571208 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15bpATnp was a gift from Keji Zhao (Addgene plasmid # 121137 ; http://n2t.net/addgene:121137 ; RRID:Addgene_121137)
  • For your References section:

    Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq). Carter B, Ku WL, Kang JY, Hu G, Perrie J, Tang Q, Zhao K. Nat Commun. 2019 Aug 20;10(1):3747. doi: 10.1038/s41467-019-11559-1. 10.1038/s41467-019-11559-1 PubMed 31431618