PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124601||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8079
- Total vector size (bp) 8679
Modifications to backboneRibosome binding site (RBS) was replaced by a canonical RBS sequence
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
- Promoter T7
/ Fusion Protein
- Protein A and Tn5 transposase fusion protein with N terminal 3XFlag tag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer CGG TTT AAA CCG GGG ATC TCG
- 3′ sequencing primer TTG CGC CGC AAC ATT CAC C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3XFlag-pA-Tn5-Fl was a gift from Steven Henikoff (Addgene plasmid # 124601 ; http://n2t.net/addgene:124601 ; RRID:Addgene_124601)
For your References section:CUT&Tag for efficient epigenomic profiling of small samples and single cells. Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. 10.1038/s41467-019-09982-5 PubMed 31036827