Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #124601)


Item Catalog # Description Quantity Price (USD)
Plasmid 124601 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8079
  • Total vector size (bp) 8679
  • Modifications to backbone
    Ribosome binding site (RBS) was replaced by a canonical RBS sequence
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
  • Alt name
  • Species
    E. coli
  • Promoter T7
  • Tag / Fusion Protein
    • Protein A and Tn5 transposase fusion protein with N terminal 3XFlag tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer CGG TTT AAA CCG GGG ATC TCG
  • 3′ sequencing primer TTG CGC CGC AAC ATT CAC C
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Tn5 transposase: pTXB1-Tn5 plasmid was a gift from Rickard Sandberg (Addgene plasmid # 60240) and for pA, the pK19pA-MN plasmid was a gift from Ulrich Laemmli (available through Addgene plasmid # 86973)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3XFlag-pA-Tn5-Fl was a gift from Steven Henikoff (Addgene plasmid # 124601 ; ; RRID:Addgene_124601)
  • For your References section:

    CUT&Tag for efficient epigenomic profiling of small samples and single cells. Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. 10.1038/s41467-019-09982-5 PubMed 31036827