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(Plasmid #124601)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124601 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 8079
  • Total vector size (bp) 8679
  • Modifications to backbone
    Ribosome binding site (RBS) was replaced by a canonical RBS sequence
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
  • Alt name
  • Species
    E. coli
  • Promoter T7
  • Tag / Fusion Protein
    • Protein A and Tn5 transposase fusion protein with N terminal 3XFlag tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer CGG TTT AAA CCG GGG ATC TCG
  • 3′ sequencing primer TTG CGC CGC AAC ATT CAC C
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Tn5 transposase: pTXB1-Tn5 plasmid was a gift from Rickard Sandberg (Addgene plasmid # 60240) and for pA, the pK19pA-MN plasmid was a gift from Ulrich Laemmli (available through Addgene plasmid # 86973)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.

This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3XFlag-pA-Tn5-Fl was a gift from Steven Henikoff (Addgene plasmid # 124601 ; ; RRID:Addgene_124601)
  • For your References section:

    CUT&Tag for efficient epigenomic profiling of small samples and single cells. Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. 10.1038/s41467-019-09982-5 PubMed 31036827

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