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165,839 results

Showing: 3161 - 3180 of 165839 results

  3. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61737-plasmid-map-sequence-id-190162

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  7. mCherry2-C1

    Plasmid
    #54563
    Purpose
    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54563-plasmid-map-sequence-id-310151

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  8. pR26 CAG/GFP Asc

    Plasmid
    #74285
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74285-plasmid-map-sequence-id-357226

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  12. mScarletI3-6DG5-MAPPER

    Plasmid
    #222421
    Purpose
    To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)
    Depositor
    Stephen Royle
    Article
    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d
    Insert
    MAPPER
    Use
    Tags
    mScarletI3-6DG5
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222421-plasmid-map-sequence-id-439142

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  13. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  16. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV2)

    Viral Prep
    #105547-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    EF1a
    Tags
    EGFP
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. pIVTRup

    Plasmid
    #101362
    Purpose
    Highly efficient in vitro transcription. Empty backbone.
    Depositor
    Ángel Raya
    Insert
    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    Use
    For cloning a gene for in vitro transcription pur…
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101362-plasmid-map-sequence-id-352564

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  18. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

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Showing: 3161 - 3180 of 165839 results