168,296 results

1. pMDC32B-NbmiR482aTS-B/c

   Plasmid

   #227967

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Inserts

   B/c
   NbmiR482aTS
   

   Expression

   Plant

   Promoter

   35S and None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-mGold2t-Lysosome-N-20

   Plasmid

   #231778

   Purpose

   Visualization of lysosome in mammalian cells using mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t-Lysosome-N-20 (Lamp1 Rat)
   

   Tags

   mGold2s

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMK195

   Plasmid

   #226818

   Purpose

   Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms

   Depositor

   David Nielsen

   Article

   Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-GST-8

   Plasmid

   #228990

   Purpose

   For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-8
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Epac-S-H326

   Plasmid

   #228242

   Purpose

   mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Epac-S-H327

   Plasmid

   #228243

   Purpose

   mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H328

   Plasmid

   #228244

   Purpose

   mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Epac-S-H329

   Plasmid

   #228245

   Purpose

   mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Epac-S-H332

   Plasmid

   #228250

   Purpose

   mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUASTattB-degradALFA

    Plasmid

    #219663

    Purpose

    Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    degradALFA
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWN172

    Plasmid

    #215291

    Purpose

    Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    J23113

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC70189

    Plasmid

    #209450

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-GRAB_g5-HT3.0mut

    Plasmid

    #208712

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p663-UBC-miniTurbo-V5-MAST3_IDG-K

    Plasmid

    #198007

    Purpose

    Gateway destination clone of MAST3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAST3 (MAST3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. BASIC_SEVA_67_pKD46.10

    Plasmid

    #178942

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Available Since

    March 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBS FGF3 isoform 1

    Plasmid

    #19230

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
    

    Expression

    Bacterial

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pmScarlet3-Peroxi_C1

    Plasmid

    #189769

    Purpose

    Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Peroxi
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid

    #203654

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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