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182,214 results

Showing: 3161 - 3180 of 182214 results
  1. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  2. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pLenti-CaMKIIa-ChETA-EYFP

    Plasmid
    #26967
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Insert
    hChR2(E123T/H134R)-EYFP
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E123T
    Promoter
    CaMKIIa
    Available since
    Jan. 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 3161 - 3180 of 182214 results