PurposeConstitutive expression of mRuby3 in bacteria
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74234||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2916
- Total vector size (bp) 3627
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)low Amp
Growth instructionsMay require extra growth time
Insert Size (bp)711
- Promoter T7
/ Fusion Protein
- 6xHis (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7 Xpress Fwd
- 3′ sequencing primer T7 term (Common Sequencing Primers)
The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.
Note that the mRuby3 insert in this plasmid does not contain T38V, like the canonical mRuby3. See publication and full plasmid sequence for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNCS-mRuby3 was a gift from Michael Lin (Addgene plasmid # 74234 ; http://n2t.net/addgene:74234 ; RRID:Addgene_74234)
For your References section:Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144