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pNCS-mRuby3
(Plasmid #74234)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74234 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNCS
  • Backbone size w/o insert (bp) 2916
  • Total vector size (bp) 3627
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    low Amp
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    May require extra growth time
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mRuby3
  • Insert Size (bp)
    711
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7 Xpress Fwd
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.

Note that the mRuby3 insert in this plasmid does not contain T38V, like the canonical mRuby3. See publication and full plasmid sequence for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNCS-mRuby3 was a gift from Michael Lin (Addgene plasmid # 74234 ; http://n2t.net/addgene:74234 ; RRID:Addgene_74234)
  • For your References section:

    Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144