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A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu YG
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
66188pYLCRISPR/Cas9Pubi-Bexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection
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66189pYLCRISPR/Cas9P35S-Hexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection
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66190pYLCRISPR/Cas9P35S-Bexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
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66191pYLCRISPR/Cas9P35S-Nexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
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66192pYLsgRNA-OsU3m/LacZcloning of sgRNAs for expression in plants
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66193pYLsgRNA-OsU3mcloning of sgRNAs for expression in plants
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66194pYLsgRNA-OsU6acloning of sgRNAs for expression in plants
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66195pYLsgRNA-OsU6a/LacZcloning of sgRNAs for expression in plants
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66196pYLsgRNA-OsU6bcloning of sgRNAs for expression in plants
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66197pYLsgRNA-OsU6ccloning of sgRNAs for expression in plants
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66198pYLsgRNA-AtU3bcloning of sgRNAs for expression in plants
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66199pYLsgRNA-AtU3b/LacZcloning of sgRNAs for expression in plants
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66200pYLsgRNA-AtU3dcloning of sgRNAs for expression in plants
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66201pYLsgRNA-AtU3d/LacZcloning of sgRNAs for expression in plants
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66202pYLsgRNA-AtU6-1cloning of sgRNAs for expression in plants
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66203pYLsgRNA-AtU6-29cloning of sgRNAs for expression in plants
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