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paGFP
(Plasmid #18697)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18697 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    photoactivatable GFP
  • Alt name
    paGFP
  • Insert Size (bp)
    700
  • Mutation
    A206K mutation
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J. Lippincott-Schwartz (paGFP)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    paGFP was a gift from Karel Svoboda (Addgene plasmid # 18697 ; http://n2t.net/addgene:18697 ; RRID:Addgene_18697)
  • For your References section:

    The spread of Ras activity triggered by activation of a single dendritic spine. Harvey CD, Yasuda R, Zhong H, Svoboda K. Science. 2008 Jul 4. 321(5885):136-40. 10.1126/science.1159675 PubMed 18556515