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mito-PAGFP
(Plasmid #23348)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23348 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mito-GFP (BD Biosciences)
  • Backbone size w/o insert (bp) 4059
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAGFP
  • Alt name
    Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase
  • Alt name
    photoactivatable mito-GFP
  • Insert Size (bp)
    741
  • Tag / Fusion Protein
    • mito (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PAGFP-N1, provided by G. Patterson and J. Lippincott-Schwartz, was used as a template for cloning the PAGFP region in place of WT GFP in the mito-GFP vector.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mito-PAGFP was a gift from Richard Youle (Addgene plasmid # 23348 ; http://n2t.net/addgene:23348 ; RRID:Addgene_23348)
  • For your References section:

    Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis. Karbowski M, Arnoult D, Chen H, Chan DC, Smith CL, Youle RJ. J Cell Biol. 2004 Feb 16. 164(4):493-9. 10.1083/jcb.200309082 PubMed 14769861