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(Plasmid #11909)


Item Catalog # Description Quantity Price (USD)
Plasmid 11909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 2 µg of cloning grade DNA in Tris buffer 1 $105

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    photoactivatable green fluorescent protein
  • Species
    Aequorea victoria
  • Mutation
    These plasmids are essentially the same as the Clontech pEGFP-N1 version with four mutations (L64F/T65S/V163A/T203H). The PAGFP also contains an A206K mutation to disrupt dimerization

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.

The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.

Information for Cloning Grade DNA (Catalog # ( Back to top )


Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.


  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPAGFP-N1 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11909 ; ; RRID:Addgene_11909)
  • For your References section:

    A photoactivatable GFP for selective photolabeling of proteins and cells. Patterson GH, Lippincott-Schwartz J. Science. 2002 Sep 13. 297(5588):1873-7. 10.1126/science.1074952 PubMed 12228718