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pBiFC-bJunVN155(I152L)
(Plasmid #27098)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27098 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMyc-CMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3791
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bJunVN155(I152L)
  • Alt name
    c-Jun(257-334)-Venus)1-154, I152L)
  • Species
    R. norvegicus (rat); and Aequorea Victoria
  • Insert Size (bp)
    689
  • Mutation
    I152L in Venus(1-154)
  • Entrez Gene
    Jun
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

To be published in Biotechniques. Please suggest users to cite the article later. Also, this plasmid can be used to replace pBiFC-bJunVN173.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiFC-bJunVN155(I152L) was a gift from Chang-Deng Hu (Addgene plasmid # 27098 ; http://n2t.net/addgene:27098 ; RRID:Addgene_27098)
  • For your References section:

    An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Nov . 49(5):793-805. 10.2144/000113519 PubMed 21091444