|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27097||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3791
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsDH5 alpha
Copy numberHigh Copy
Alt nameVenus (1-154, I152L)
Insert Size (bp)462
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
To be published by Biotechniques in December. Please suggest the citation of our Biotechniques article. Also, this will be used as a replacement of pBiFC-VN173.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBiFC-VN155(I152L) was a gift from Chang-Deng Hu (Addgene plasmid # 27097 ; http://n2t.net/addgene:27097 ; RRID:Addgene_27097)
For your References section:An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Nov . 49(5):793-805. 10.2144/000113519 PubMed 21091444