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pHsp70-Cas9 Citations (16)

Originally described in: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.
Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM Genetics. 2013 May 24.
PubMed Journal

Articles Citing pHsp70-Cas9

Cas9-based genome editing in Drosophila. Housden BE, Lin S, Perrimon N. Methods Enzymol. 2014;546:415-39. doi: 10.1016/B978-0-12-801185-0.00019-2. PubMed
Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti. Dong S, Lin J, Held NL, Clem RJ, Passarelli AL, Franz AW. PLoS One. 2015 Mar 27;10(3):e0122353. doi: 10.1371/journal.pone.0122353. eCollection 2015. PubMed
Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations. Champer J, Reeves R, Oh SY, Liu C, Liu J, Clark AG, Messer PW. PLoS Genet. 2017 Jul 20;13(7):e1006796. doi: 10.1371/journal.pgen.1006796. eCollection 2017 Jul. PubMed
CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity. Xu XS, Gantz VM, Siomava N, Bier E. Elife. 2017 Dec 23;6. pii: 30281. doi: 10.7554/eLife.30281. PubMed
Reducing resistance allele formation in CRISPR gene drive. Champer J, Liu J, Oh SY, Reeves R, Luthra A, Oakes N, Clark AG, Messer PW. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5522-5527. doi: 10.1073/pnas.1720354115. Epub 2018 May 7. PubMed
Molecular safeguarding of CRISPR gene drive experiments. Champer J, Chung J, Lee YL, Liu C, Yang E, Wen Z, Clark AG, Messer PW. Elife. 2019 Jan 22;8. pii: 41439. doi: 10.7554/eLife.41439. PubMed
Maximum Likelihood Estimation of Fitness Components in Experimental Evolution. Liu J, Champer J, Langmuller AM, Liu C, Chung J, Reeves R, Luthra A, Lee YL, Vaughn AH, Clark AG, Messer PW. Genetics. 2019 Mar;211(3):1005-1017. doi: 10.1534/genetics.118.301893. Epub 2019 Jan 24. PubMed
Efficient allelic-drive in Drosophila. Guichard A, Haque T, Bobik M, Xu XS, Klanseck C, Kushwah RBS, Berni M, Kaduskar B, Gantz VM, Bier E. Nat Commun. 2019 Apr 9;10(1):1640. doi: 10.1038/s41467-019-09694-w. PubMed
A toxin-antidote CRISPR gene drive system for regional population modification. Champer J, Lee E, Yang E, Liu C, Clark AG, Messer PW. Nat Commun. 2020 Feb 27;11(1):1082. doi: 10.1038/s41467-020-14960-3. PubMed
Genetic transformation of mosquitoes by microparticle bombardment. Lule-Chavez AN, Carballar-Lejarazu R, Cabrera-Ponce JL, Lanz-Mendoza H, Ibarra JE. Insect Mol Biol. 2021 Feb;30(1):30-41. doi: 10.1111/imb.12670. Epub 2020 Oct 26. PubMed
DNA polymerase theta suppresses mitotic crossing over. Carvajal-Garcia J, Crown KN, Ramsden DA, Sekelsky J. PLoS Genet. 2021 Mar 22;17(3):e1009267. doi: 10.1371/journal.pgen.1009267. eCollection 2021 Mar. PubMed
Loss of UCHL1 rescues the defects related to Parkinson's disease by suppressing glycolysis. Ham SJ, Lee D, Xu WJ, Cho E, Choi S, Min S, Park S, Chung J. Sci Adv. 2021 Jul 9;7(28). pii: 7/28/eabg4574. doi: 10.1126/sciadv.abg4574. Print 2021 Jul. PubMed
A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles. Yang E, Metzloff M, Langmuller AM, Xu X, Clark AG, Messer PW, Champer J. G3 (Bethesda). 2022 May 30;12(6). pii: 6565321. doi: 10.1093/g3journal/jkac081. PubMed
Experimental demonstration of tethered gene drive systems for confined population modification or suppression. Metzloff M, Yang E, Dhole S, Clark AG, Messer PW, Champer J. BMC Biol. 2022 May 24;20(1):119. doi: 10.1186/s12915-022-01292-5. PubMed
Copy number changes in co-expressed odorant receptor genes enable selection for sensory differences in drosophilid species. Auer TO, Alvarez-Ocana R, Cruchet S, Benton R, Arguello JR. Nat Ecol Evol. 2022 Sep;6(9):1343-1353. doi: 10.1038/s41559-022-01830-y. Epub 2022 Jul 21. PubMed
Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations. Langmuller AM, Champer J, Lapinska S, Xie L, Metzloff M, Champer SE, Liu J, Xu Y, Du J, Clark AG, Messer PW. Elife. 2022 Sep 22;11:e71809. doi: 10.7554/eLife.71809. PubMed

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