|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32640||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTetanus toxin light chain
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Addgene's sequencing results identified single nucleotide insertions at bp# 2955/2956 and 4357/4358, as well as a 3 nucleotide insertion at bp# 4344/4345, when compared to the author's sequence. The insertions are outside of the coding region and should not be a concern for the intended purpose of the plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEMTEZ-TeTxLC was a gift from Richard Axel & Joseph Gogos & C. Ron Yu (Addgene plasmid # 32640 ; http://n2t.net/addgene:32640 ; RRID:Addgene_32640)
For your References section:Spontaneous neural activity is required for the establishment and maintenance of the olfactory sensory map. Yu CR, Power J, Barnea G, O'Donnell S, Brown HE, Osborne J, Axel R, Gogos JA. Neuron. 2004 May 27;42(4):553-66. 10.1016/S0896-6273(04)00224-7 PubMed 15157418