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(Plasmid #12433)


Item Catalog # Description Quantity Price (USD)
Plasmid 12433 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    See comments
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    AML1-ETO9a fusion
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    AML-ETO protein with ETO exon 9a.
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
  • Entrez Gene
    RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".

We used the oligonucleotides 5'-GTTTGGATGA TATGGCCATTG-3' and 5'-CCATCGATCCTCA TATGACCCAGGACAGC-3' in PCR with cDNA from Kasumi-1 cells to generate a DNA fragment from the BamHI site of ETO to the ETO9a coding sequence including the stop codon. We subcloned this fragment into the pGEM vector (Promega) and subsequently inserted it into the BamHI and EcoRI sites of MigR1-AE to form MigR1-AE9a.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MigR1-AE9a was a gift from Dong-Er Zhang (Addgene plasmid # 12433 ; ; RRID:Addgene_12433)
  • For your References section:

    A previously unidentified alternatively spliced isoform of t(8;21) transcript promotes leukemogenesis. Yan M, Kanbe E, Peterson LF, Boyapati A, Miao Y, Wang Y, Chen IM, Chen Z, Rowley JD, Willman CL, Zhang DE. Nat Med. 2006 Aug . 12(8):945-9. 10.1038/nm1443 PubMed 16892037