|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12433||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerSee comments
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameAML1-ETO9a fusion
SpeciesH. sapiens (human)
MutationAML-ETO protein with ETO exon 9a.
Entrez GeneRUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
Entrez GeneRUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".
We used the oligonucleotides 5'-GTTTGGATGA TATGGCCATTG-3' and 5'-CCATCGATCCTCA TATGACCCAGGACAGC-3' in PCR with cDNA from Kasumi-1 cells to generate a DNA fragment from the BamHI site of ETO to the ETO9a coding sequence including the stop codon. We subcloned this fragment into the pGEM vector (Promega) and subsequently inserted it into the BamHI and EcoRI sites of MigR1-AE to form MigR1-AE9a.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MigR1-AE9a was a gift from Dong-Er Zhang (Addgene plasmid # 12433 ; http://n2t.net/addgene:12433 ; RRID:Addgene_12433)
For your References section:A previously unidentified alternatively spliced isoform of t(8;21) transcript promotes leukemogenesis. Yan M, Kanbe E, Peterson LF, Boyapati A, Miao Y, Wang Y, Chen IM, Chen Z, Rowley JD, Willman CL, Zhang DE. Nat Med. 2006 Aug . 12(8):945-9. 10.1038/nm1443 PubMed 16892037