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MigR1-AE
(Plasmid #12431)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12431 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MigR1
  • Backbone manufacturer
    See comments
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AML1-ETO fusion
  • Alt name
    AML1
  • Alt name
    ETO
  • Species
    H. sapiens (human)
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
  • Entrez Gene
    RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site unknown (not destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer pSIN-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    (HA)-tagged AML1-ETO was a gift from M. Ohki, National Cancer Center Research Institute, Tokyo.

Terms and Licenses

Depositor Comments

MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MigR1-AE was a gift from Dong-Er Zhang (Addgene plasmid # 12431 ; http://n2t.net/addgene:12431 ; RRID:Addgene_12431)
  • For your References section:

    Deletion of an AML1-ETO C-terminal NcoR/SMRT-interacting region strongly induces leukemia development. Yan M, Burel SA, Peterson LF, Kanbe E, Iwasaki H, Boyapati A, Hines R, Akashi K, Zhang DE. Proc Natl Acad Sci U S A. 2004 Dec 7. 101(49):17186-91. 10.1073/pnas.0406702101 PubMed 15569932