|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20873||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namemixed-lineage leukemia
Alt nameENL, mixed lineage leukemia eleven nineteen leukemia
SpeciesH. sapiens (human)
Insert Size (bp)4580
Entrez GeneKMT2A (a.k.a. ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1, MLL1A, TRX1, WDSTS)
Entrez GeneMLLT1 (a.k.a. ENL, LTG19, YEATS1)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer MSCV
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
This is a control construct testing the newly developed fMLLpl construct for transforming activity in a MLL-ENL fusion.
SmaI/XhoI fragment from pBluescriptII-ENL3' was inserted into a PmlI/XhoI cut pMSCV-flagMLLpl vector. The end plasmid has ~4.18kb MLL + pl remainings + ~0.4kb ENL with remaining pBSII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-FlagMLL-pl-ENL(5613) was a gift from Robert Slany (Addgene plasmid # 20873 ; http://n2t.net/addgene:20873 ; RRID:Addgene_20873)
For your References section:Alterations of the CxxC domain preclude oncogenic activation of mixed-lineage leukemia 2. Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK. Oncogene. 2009 Feb 12. 28(6):815-23. 10.1038/onc.2008.443 PubMed 19060922