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(Plasmid #20873)


Item Catalog # Description Quantity Price (USD)
Plasmid 20873 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    mixed-lineage leukemia
  • Alt name
    ENL, mixed lineage leukemia eleven nineteen leukemia
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    KMT2A (a.k.a. ALL-1, ALL1, CXXC7, GAS7, HRX, HTRX, HTRX1, MLL, MLL1, MLL1A, TRX1, WDSTS)
  • Entrez Gene
    MLLT1 (a.k.a. ENL, LTG19, YEATS1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a control construct testing the newly developed fMLLpl construct for transforming activity in a MLL-ENL fusion.

SmaI/XhoI fragment from pBluescriptII-ENL3' was inserted into a PmlI/XhoI cut pMSCV-flagMLLpl vector. The end plasmid has ~4.18kb MLL + pl remainings + ~0.4kb ENL with remaining pBSII.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-FlagMLL-pl-ENL(5613) was a gift from Robert Slany (Addgene plasmid # 20873 ; ; RRID:Addgene_20873)
  • For your References section:

    Alterations of the CxxC domain preclude oncogenic activation of mixed-lineage leukemia 2. Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK. Oncogene. 2009 Feb 12. 28(6):815-23. 10.1038/onc.2008.443 PubMed 19060922