Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMSCV-FlagMLL-pl-ENL(5613)
(Plasmid #20873)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20873 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCV-neo
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLL
  • Alt name
    mixed-lineage leukemia
  • Alt name
    ENL, mixed lineage leukemia eleven nineteen leukemia
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4580
  • Entrez Gene
    KMT2A (a.k.a. ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1, MLL1A, TRX1, WDSTS)
  • Entrez Gene
    MLLT1 (a.k.a. ENL, LTG19, YEATS1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Francis Stewart, University Durden Germany
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

This is a control construct testing the newly developed fMLLpl construct for transforming activity in a MLL-ENL fusion.

SmaI/XhoI fragment from pBluescriptII-ENL3' was inserted into a PmlI/XhoI cut pMSCV-flagMLLpl vector. The end plasmid has ~4.18kb MLL + pl remainings + ~0.4kb ENL with remaining pBSII.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-FlagMLL-pl-ENL(5613) was a gift from Robert Slany (Addgene plasmid # 20873 ; http://n2t.net/addgene:20873 ; RRID:Addgene_20873)
  • For your References section:

    Alterations of the CxxC domain preclude oncogenic activation of mixed-lineage leukemia 2. Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK. Oncogene. 2009 Feb 12. 28(6):815-23. 10.1038/onc.2008.443 PubMed 19060922