|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21114||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1(+) (Invitrogen)
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)434
Entrez GeneMIR21 (a.k.a. MIRN21, hsa-mir-21, miR-21, miRNA21)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-miR21 was a gift from Joshua Mendell (Addgene plasmid # 21114 ; http://n2t.net/addgene:21114 ; RRID:Addgene_21114)
For your References section:Cell-cell contact globally activates microRNA biogenesis. Hwang HW, Wentzel EA, Mendell JT. Proc Natl Acad Sci U S A. 2009 Apr 9. ():. 10.1073/pnas.0811523106 PubMed 19359480