|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20381||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3200
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)93
Entrez GeneMIR21 (a.k.a. MIRN21, hsa-mir-21, miR-21, miRNA21)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV promoter (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-miR21 was a gift from Bryan Cullen (Addgene plasmid # 20381 ; http://n2t.net/addgene:20381 ; RRID:Addgene_20381)
For your References section:Sequence requirements for micro RNA processing and function in human cells. Zeng Y, Cullen BR. RNA. 2003 Jan . 9(1):112-23. 10.1261/rna.2780503 PubMed 12554881
Map uploaded by the depositor.