PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||139054||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Promoter UBC
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcorI (not destroyed)
- 5′ sequencing primer TGAAGCTCCGGTTTTGAACT (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLJC5-3XHA-EGFP-PEX26 was a gift from David Sabatini (Addgene plasmid # 139054 ; http://n2t.net/addgene:139054 ; RRID:Addgene_139054)
For your References section:A PEROXO-Tag enables rapid isolation of peroxisomes from human cells. Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW, Sabatini DM. iScience, ISSN: 2589-0042, Vol: 0, Issue: 0, Page: 101109 10.1016/j.isci.2020.101109