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Multimodal fast optical interrogation of neural circuitry.
Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K
Nature. 2007 Apr 5. 446(7136):633-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
20938pcDNA3.1/hChR2(H134R)-mCherryMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
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20939pcDNA3.1/hChR2-EYFPMammalian expression of humanized ChR2 fused to EYFP
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20940pcDNA3.1/hChR2(H134R)-EYFPMammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
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20942pLenti-EF1a-hChR2(H134R)-EYFP-WPRE3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation
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20943pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation
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20944pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation
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20945pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation
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20946pLenti-CaMKIIa-eNpHR-EYFP-WPRE3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition
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