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pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
(Plasmid #20945)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20945 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-SynapsinI
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    channelrhodopsin-2
  • Alt name
    ChR2
  • Alt name
    cop4
  • Alt name
    channelopsin-2
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1662
  • Mutation
    H134R mutation in ChR2
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see http://www.optogenetics.org

for additional information.

The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE was a gift from Karl Deisseroth (Addgene plasmid # 20945 ; http://n2t.net/addgene:20945 ; RRID:Addgene_20945)
  • For your References section:

    Multimodal fast optical interrogation of neural circuitry. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K. Nature. 2007 Apr 5. 446(7136):633-9. 10.1038/nature05744 PubMed 17410168